Indication
Diagnosis Cushings syndrome

Preparation
No food or fluids 30 minutes prior to the collection of each sample.

Procedure
1. Saliva samples can be collected with either a salivette or manually.
2. Collect 1 sample between 11 PM and midnight
3. It is imperative that each sample has a sufficient amount of saliva for accurate interpretation.
4. All collection tubes must have the name of patient and physician, date and time.
5. Return the samples immediately to the physicians office after the test is complete.
6. Saliva samples are then sent to an appropriate laboratory for analysis.

Interpretation
A normal variation of salivary cortisol levels suggest the patient does not have Cushing’s syndrome. The failure of cortisol variation throughout the day suggests the presence of hypercortisolism warranting further medical evaluation.